Top Down Mass Spectrometry of Archaea and Human Tumor Cells

A final study was performed applying the top down platform to human HeLa tumor cells. Thirty-four proteins in all were identified, and >90% of these were fully characterized. Several proteins harbored post-translational modifications including N-terminal and internal acetylations, methylations, disulfide bonds, and protein truncations. Proteins were also characterized with known coding single nucleotide polymorphisms, alternative splice variants, and highly homologous gene family members.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Forbes, Andrew James

Contributors dc:contributor

• Kelleher, Neil L.

• Chemistry, Analytical

Language dc:language

eng

Identifier

(MiAaPQ)AAI3153294

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84151