Part I. The Synthesis of Hyaluronan Oligosaccharides and the Mild Cleavage of 2-Amino-2-Deoxy-D-Glucoside Methoxycarbonyl Derivatives With Methytrichlorosilane. Part II. The Design and Engineering of a Manganese Binding Site in Cytochrome C Peroxidase

Part II. A manganese binding site has been creating in CcP corresponding to the Mn-binding domain in MnP by site directed mutagenesis. Several spectroscopic techniques including paramagnetic NMR and EPR were employed to characterize the CcP mutant (MnCcP). The data is consistent with a newly formed metal site that binds a single Mn2+ near the heme periphery. Wild-type CcP exhibits none of the spectroscopic features as MnCcP. The new binding site provides a five-fold increase in Mn-oxidizing activity in MnCcP over that of WTCcP.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Yeung, Bryan K.S.

Contributors dc:contributor

• Petillo, Peter A.

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(MiAaPQ)AAI3017260

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84032