Biochemical and Genetic Studies on Origin Recognition and DNA Synthesis From the Mesophilic Archaeon Methanosarcina Acetivorans

In our study, we characterized DinB and found that DinB was able to synthesize through abasic DNA and cyclobutane pyrimidine dimers, and these activities were stimulated by the cognate PCNA. Fascinatingly, unlike most translesion DNA polymerases previously described, MacDinB synthesized an unusually long product (∼7.2 kb) in the presence of the sliding clamp. A PCNA-interacting peptide (PIP) box located at the C-terminus of MacDinB was shown to facilitate interaction with the sliding clamp. Furthermore, individual mutations at different positions in the PIP-box led to identification of residues that are critical to PCNA/DinB interactions. Nevertheless, the biochemical analysis described here for DinB provides important insights into the properties, potential, and diversity of the Y-family of DNA polymerases.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Animal Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Lin, Li-Jung

Contributors dc:contributor

• Isaac Cann

• Biology, Microbiology

Language dc:language

eng

Identifier

(MiAaPQ)AAI3392194

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/83627