Structural Transitions in Self-Assembled Lipid Systems Driven by Induced Curvature: From Cell -Penetrating Peptides to Programmable Vesicles

We also examined the interactions of other arginine rich cell-penetrating peptides, including Antp and oligoarginine, with model cell membranes, and find that the transduction activity correlates with induction of negative Gaussian curvature. The negative Gaussian membrane curvature is broadly enabling and its induction can lower the free energy barriers for a range of different entry mechanisms, such as direct translocation as well as endocytotic pathways. Furthermore, we show that the TAT peptide interacts strongly with actin cytoskeleton, which enhances membrane deformation and cytoskeleton reorganization necessary for endocytotic processes. We propose a mechanism that explains how a relatively simple molecule, like the TAT peptide, facilitates direct entry and multiple endocytotic mechanisms.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Materials Science and Engineering

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Mishra, Abhijit

Contributors dc:contributor

• Gerard Wong

• Biophysics, General

Language dc:language

eng

Identifier

(MiAaPQ)AAI3431017

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/82865