Development of a Fluorescence Lifetime Based Method to Detect and Analyze Single Molecule Reactions in Solution

This new hardware was used to observe the conformational dynamics of a calmodulin sample labeled with a FRET pair, and encapsulated within 100nm lipid vesicles. A toolbox of analysis techniques was developed for PTA, and was used to quantitatively describe the transitions and fluctuations of the conformation of calmodulin. Analysis was done in a model-free manner, and also by applying known parameters about the system to extract more specific information. Using the information obtained, a conformational model was developed to describe the dynamic behavior of calmodulin's conformation in terms of its binding with calcium. In addition, measurements conducted in the presence of a peptide derived from Ca2+/calmodulin-dependent protein kinase II were used to examine the properties of calmodulin's conformational dynamics while interacting with its binding targets.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Physics

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Colyer, Ryan Anthony

Contributors dc:contributor

• Gratton, Enrico

• Biophysics, General

Language dc:language

eng

Identifier

(MiAaPQ)AAI3337743

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/80576