Lighting Up Life: Fluorescence Studies of the Shaker Potassium Ion Channel and Myosin v in Action

"The other protein I studied is myosin V which walks bipedally along actin filaments taking steps which displace the center of mass 37 nm per step. A controversy over the nature of this movement was settled earlier this year by other members of our laboratory when they showed that myosin V processes in a hand-over-hand manner, with each of the heads alternating in the leading and trailing positions. To do this, they developed a new technique which they called FIONA for Fluorescence Imaging with One Nanometer Accuracy which allowed them to localize individual molecules of myosin V to within 1.5 nut on the surface of a microscope cover slip. I have extended and applied FIONA to a mutant of myosin V with a molecule of the enhanced Green Fluorescent Protein (eGFP) fused to one head of the dieter. I demonstrated that eGFP is suitable to use with FIONA, confirm the hand-over-hand model of myosin procession, and argue that in aggregate, the data on myosin's steps from our lab implies that myosin V adopts an asymmetric, ""telemark skier,"" configuration between steps. (Abstract shortened by UMI.)."

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Physics

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Snyder, Gregory E.

Contributors dc:contributor

• Selvin, Paul R.

• Biophysics, General

Language dc:language

eng

Identifier

(MiAaPQ)AAI3101972

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/80497