Mueller-matrix-based analysis for second-harmonic generation imaging

Second-harmonic generation microscopy takes advantage of second-order nonlinear property for imaging of biological structures. Invoking quantitative image analysis techniques facilitates the assessment of tissues in varying states. This thesis presents a quantitative description of the relative structural difference in collagen-based tissues by second harmonic generation (SHG) imaging techniques involving polarization investigation. The Mueller matrix formalism demonstrates a polarization-based analysis approach for assessment of varying samples. The associated second-order Mueller matrices are generated by applying image processing algorithms to sub-grids of image sets obtained from a forward imaging microscope. By defining scalar metrics such as the degree of polarization and the depolarization, a quantitative evaluation of the samples under investigation is provided. A discussion of the capabilities for tissue assessment and techniques for improvements in the data acquisition speed and analysis will also be proffered

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Electrical & Computer Engr

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Okoro, Chukwuemeka Onyekere

• Second-harmonic generation
• Mueller matrix

Statement dc:rights

• Copyright 2015 Chukwuemeka Okoro

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/78681

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/78681