Biochemical Characterizations of a Soybean (Glycine Max (L.) Merr. ) Nitrate Reductase Mutant and Crude and Purified Nitrate Reductases

Wild-type soybean Glycine max (L.) Merr. cv. Williams leaves contain three forms of nitrate reductase (NR), two functional in the absence of NO(,3)('-) in the growth medium (constitutive forms; c(,1)NR, c(,2)NR) and the third active after induction by NO(,3)('-) (inducible form; iNR). Experiments were conducted to determine the site of effect of a mutation which resulted in loss of cNR activity in the soybean mutant (gene symbol nr(,1)). In addition, crude and purified preparations of soybean leaf NR forms were biochemically characterized.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Botany

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Nelson, Richard Scott

• Biology, Plant Physiology

Language dc:language

eng

Identifier

(UMI)AAI8521847

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/77660