"The use of retinoids and an in vivo ""affinophoresis"" assay to analyze the cellular basis of positional memory in regenerating axolotl limbs"

Amphibian limb regeneration blastema cells normally regenerate only those parts distal to the amputation plane, and have been shown to inherit an expressed memory of their position of origin, termed positional value, by developing autonomously when grafted away from their normal limb site, and by inducing intercalary regeneration from more proximal limb stumps. Recently, retinoids (vitamin A and its derivatives) have been shown to predictably modify positional memory in regenerating amphibian limbs resulting in PD duplications of stump structures within the regenerate. This discovery creates a means by which the cellular and molecular basis of pattern formation can be investigated. This study describes an in vivo assay that was devised to detect level-specific differences in cell affinity along the proximodistal (PD) axis of regenerating axolotl limbs, and several experiments employing retinoic acid (RA) designed to correlate these differences with positional memory.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Crawford, Karen

Contributors dc:contributor

• Stocum, David L.,

• Biology, General

Language dc:language

eng

Identifier

(UMI)AAI8803009

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/77648