Structural Characterization of the Transfer Ribonucleic Acids From Halobacterium Volcanii and Other Archaebacteria

The archaebacteria have been defined primarily on the basis of their ribosomal RNAs, as a primary kingdom, distinct from the eubacteria ('prokaryotes') and the eucaryotes. To help in evaluating the molecular affinities, we characterize here the transfer RNAs (tRNAs) in two ways. First, we describe the modification patterns of the tRNAs of ten archaebacteria (Halobacterium volcanii, Halococcus morrhuae, Methanobacterium bryantii, Methanobrevibacter smithii, Methanococcus vannielii, Methanococcus voltae, Methanomicrobium mobile, Methanosarcina barkeri, Thermoplasma acidophilum, and Sulfolobus acidocaldarius), as analyzed by two-dimensional thin layer chromatography of the ('32)P-labelled nucleotides. Second, we describe the sequences of seventeen H. volcanii tRNAs (tentative for two) as determined by the oligonucleotide cataloging methods. These sequences have been largely confirmed by sequencing 3'-end labelled tRNAs by gel methods.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Gupta, Ramesh

• Biology, Microbiology

Language dc:language

eng

Identifier

(UMI)AAI8114429

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/77583