Influence of Dietary Arginine Deficiency and Increased Nitrogen Metabolism on the Induction of Orotic Aciduria

Arginine deficiency is associated with a dramatic orotic aciduria. Liver slices from rats fed a purified L-amino acid diet with (control) and without arginine supplementation were used for studies of ('14)C-bicarbonate incorporation into orotic acid. The nanomoles of orotic acid synthesized in isolated liver slices from both control and arginine deficient animals increased linearly with time. Orotic acid biosynthesis was significantly greater in liver slices than slices of heart, muscle, kidney, and minced spleen when ammonia was used as a nitrogen source. The order of orotate biosynthesis from ('14)C-bicarbonate was liver > spleen > kidney > muscle > heart. Arginine deficiency resulted in a significant stimulation of liver orotic acid biosynthesis. Orotic acid synthesis from spleens isolated from arginine deficient rats was also enhanced compared with controls. Orotic acid production in liver slices using glutamine as the nitrogen source was also significantly greater in rats fed an arginine deficient diet compared to controls.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Food Science

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Hassan, Abdelmonem Sadig

• Health Sciences, Nutrition

Language dc:language

eng

Identifier

(UMI)AAI8026514

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/77248