Moessbauer Studies on Partially Oriented Samples of Carbon Monoxymyoglobin and Photodissociated Carbon Monoxymyoglobin

('57)Fe studies on partially oriented samples of carbon monoxy myoglobin (MbCO) and photodissociated carbon monoxy myoglobin (Mb*CO) are reported. The partial orientation was achieved by photodissociating MbCO utilizing the heme group's optical anisotropy, high quantum efficiency, and the slow recombination rate of Mb*CO at 4.2(DEGREES)K. The spectrum of each Mb species consists of a doublet which is the result of the quadrupole interaction with the electric field gradient (EFG) at the ('57)Fe nucleus. The photo-produced partial orientation affects the intensity ratio R of the two lines; measurements of R versus the fraction MbCO remaining allows the determination of the asymmetry parameter (eta) of the EFG and the angle (psi) between its principal axis V(,zz) and the normal to the optical axis, which coincides with the heme normal. The goal of this study was to demonstrate the feasibility of such an experiment by measuring (eta) and (psi) for MbCO and Mb*CO.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Physics

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Emberson, Richard Maury, Jr.

• Physics, Condensed Matter

Language dc:language

eng

Identifier

(UMI)AAI8127586

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/77205