The Isolation and Evaluation of Biological Effects of the Glucosinolate Hydrolysis Product 1-Isothiocyanato-3-(methylsulfinyl)-Propane

1-Isothiocyanato-3-(methylsulfinyl)-propane (IMSP) is a glucosinolate hydrolysis product formed from 3-methylsulfinylpropylglucosinolate when crucifers are eaten. The four most commonly consumed crucifers (cabbage, Brussels sprouts, cauliflower, and broccoli) have been shown to contain high concentrations of 3-methylsulfinylpropylglucosinolate, resulting in an estimated mean human intake of IMSP of 1 μmol/kg/day. Due to difficulties in obtaining purified compounds, methods were developed for the high yield purification of IMSP and related compounds from Lesquerella and broccoli seeds. The procedure used solvent extraction of autolyzed defatted seed meals, followed by hydrolysis product purification using gel filtration chromatography and reversed-phase HPLC.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Veterinary Medical Science

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Kore, Anita Maureen

Contributors dc:contributor

• Beasley, V.R.,

• Health Sciences, Toxicology
• Agriculture, Animal Culture and Nutrition
• Health Sciences, Nutrition

Identifier

(UMI)AAI9305588

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/72556