Characterization and Analysis of Anti-Metatype Antibodies

Immunizations of high affinity anti-fluorescein monoclonal antibody 4-4-20 affinity-labeled with fluorescein 5-isothiocyanate into rabbit elicited antibodies specific for the liganded conformation of 4-4-20. Termed anti-metatype antibodies, this polyclonal reagent also demonstrated the unique ability to delay the rate of dissociation of fluorescyl ligand from the active site of 4-4-20. This delay led to an artificial enhancement of the affinity of 4-4-20 for fluorescyl ligand. Utilization of a single chain derivative of 4-4-20 confirmed that anti-metatype reactivity was variable-region specific. Additionally, anti-metatype antibodies reacted with members of the 4-4-20 idiotype family, demonstrating varying degrees of affinity enhancement with idiotypically related molecules.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Weidner, Karla Marie

Contributors dc:contributor

• Voss, Edward W., Jr.

• Chemistry, Biochemistry
• Health Sciences, Immunology

Identifier

(UMI)AAI9305728

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/72524