Overexpression, Purification and Characterization of Recombinant Human Phosphoribosylpyrophosphate Synthetase Isozymes I and II

Phosphoribosylpyrophosphate (PRPP) synthetase catalyzes the formation of PRPP, an essential substrate in the biosynthesis of purine, pyrimidine and pyridine nucleotides. At least three isozymes of mammalian PRPP synthetase exist, and the corresponding genes are differentially expressed in tissues, suggesting the existence of functional differences in catalytic and/or regulatory properties between these isozymes. Previous studies of human PRPP synthetase were carried out prior to knowledge of the existence of multiple isozymes.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Nosal, Jane Marie

Contributors dc:contributor

• Switzer, Robert L.

• Biology, Molecular
• Chemistry, Biochemistry

Identifier

(UMI)AAI9411734

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/72363