Structural Studies of DNA Replication Machinery in Archaea

DNA replication is a fundamental process present in all living organisms. It is the means by which organisms transfer their genetic information to their offspring to ensure the propagation of their lineage. One of the essential features of DNA replication is to separate the DNA double helix to generate a replication fork by exposing two single strands that serve as templates. Studies on the replication fork have identified large number of proteins involved in this process. The underlying mechanisms of DNA replication has been intensively studied in both bacteria and eukaryotes. However, DNA replication in archaea, the third domain of life, is not well characterized. Accumulating evidence indicates that archaeal DNA replication machinery is more homologous to that of eukaryotes but presents a simpler molecular architecture. The aim of my thesis work is to use biochemical and biophysical methods to investigate the molecular basis of structure-function relationship, protein-protein interaction, and protein-DNA interaction in the archaeal DNA replication machinery.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Bae, Brian Bongik

Contributors dc:contributor

• Nair, Satish K.

• Chemistry, Biochemistry

Identifier

(UMI)AAI3399012

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/72344