Evolution of (Beta/alpha)8 Barrels: Refinement of Enzymatic Efficiency for a New Ortho-Succinylbenzoate Synthase (Osbs) From a Promiscuous Progenitor

This work allowed a step-wise pathway of changing base pairs to be elucidated that illustrates that a minimal amount of mutations (four from wild-type to I19F/R24W/D297G) are necessary to develop a new function. Additionally, the "new" function of OSBS activity was achieved with changes to substrate specificity, the substitutions allowed the catalytic barrel to remain "hard-wired" to perform acid/base chemistry as both the AEE and OSBS reactions utilized the same catalytic lysines.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Vick, Jacob E.

Contributors dc:contributor

• Gerlt, John A.

• Chemistry, Biochemistry

Identifier

(UMI)AAI3314925

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/72334