Capillary Electrophoretic Technologies for Single Cell Metabolomics

Understanding the functioning of the brain is hindered by a lack of knowledge of the full complement of neurotransmitters and neuromodulatory compounds. Single cell measurements aid in the discovery of neurotransmitters used by small subsets of neurons that would be diluted below detection limits or masked by ubiquitous compounds when working with larger brain regions. Also, as neurochemistry can be different even in adjacent neurons, single cell measurements allow a unique perspective on cell-cell signaling in the brain. Here several instrument platforms have been created that combine capillary electrophoresis (CE) with information-rich detection methods in order to perform single cell measurements. CE is an appropriate separation technique because of its compatibility with the small volumes of the cellular samples, as well as its ability to carry out online sample enrichment.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Lapainis, Theodore E.

Contributors dc:contributor

• Sweedler, Jonathan V.

• Chemistry, Analytical

Identifier

(UMI)AAI3392110

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/72255