Genetic Analysis of Clostridium Acetobutylicum Using Two-Dimensional PFGE to Construct a Macrorestriction Map of ATCC 824 and Restriction Enzyme Profiles for Strain Analysis

C. acetobutylicum and C. butyricum strains were analyzed using rare-cutting restriction enzymes by one-dimensional and two-dimensional PFGE. C. acetobutylicum strains were placed into three groups based on their restriction enzyme profiles following digestion with SalI, SmaI, and MluI. C. acetobutylicum strains ATCC 824, NCIB 8052, and NRRL B-591 were grouped together. A second group was composed of C. acetobutylicum strains ATCC 3179 and NRRL 4259. C. acetobutylicum P262 and C. butyricum NRRL B-593 were placed in individual groups. The four Clostridia groups displayed very low restriction enzyme profile similarities. The C. acetobutylicum ATCC 824 chromosome was further characterized with a macrorestriction map consisting of 92 restriction sites and seventeen genes. The macrorestriction map was constructed using combinations of SalI, SmaI, and MluI to digest the chromosomal DNA followed by two-dimensional PFGE.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Food Science

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Broussard, Suzanne Rene

Contributors dc:contributor

• Blaschek, Hans,

• Biology, Molecular
• Biology, Microbiology

Identifier

(UMI)AAI9503148

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/72226