Proteolytic Activity of Ruminal Bacteria: Studies With Butyrivibrio Fibrisolvens (Protease, Anaerobe, Protein)

The proteolytic activity of the ruminal anaerobe Butyrivibrio fibrisolvens was examined using azocasein degradation to monitor activity. Growing cells of B. fibrisolvens produced an extracellular proteolytic activity and the rate of enzyme synthesis largely paralleled growth. Protease production was not subject to induction as enzyme activity was always present regardless of the nitrogen source or carbohydrate source provided in the growth medium. Production of proteolytic activity may be subject to repression as ammonia (10 mM) in the presence of peptides (Trypticase, 1 g/l) or high concentrations of peptides (Tryticase, 20 g/l) alone caused approximately a 50% depression in the production of activity. Cultures provided with different carbohydrate sources for growth exhibited differences in protease production and the production of activity was positively correlated with growth rate.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Dairy Science

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Cotta, Michael Anthony

• Biology, Microbiology

Identifier

(UMI)AAI8511599

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/71725