Evolution of Nitrogen Oxide(s) From Soybean and the Association of This Activity to the Constitutive Nad(p)h-Nitrate Reductase Enzyme

Gas chromatography and mass spectrometry were used to determine the identity and the quantity of volatile N products produced during the helium-purged in vivo nitrate reductase (NR) assay of soybean (Glycine max (L.) Merr. cv Williams) and winged bean (Psophocarpus tetragonolobus (L.) DC. cv Lunita) leaflets. Nitric oxide (NO) and nitrous oxide (N$\sb2$O) were identified as the main N products formed. NO and N$\sb2$O produced from soybean leaflets were both labeled with $\sp $N when $\sp $N-nitrate was used in the assay medium, demonstrating that both were produced from nitrate during nitrate reduction. Leaves of winged bean, subjected to the purged in vivo NR assay, evolved greater quantities of NO and N$\sb2$O than did soybean. In both species NO production was dominant. In contrast, with similar assays, NO and N$\sb2$O were not evolved from leaves of the nr$\sb1$ soybean mutant which lacks the constitutive NR enzymes.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Agronomy

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Dean, John Vahle

Contributors dc:contributor

• Harper, James E.

• Agriculture, Agronomy
• Biology, Plant Physiology

Identifier

(UMI)AAI8815334

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/71647