Characterization of Luteinizing Hormone - Releasing Hormone (Lhrh) Release From Rat Hypothalamus Using an in Vitro System

The effect of a variety of experimental compounds on the release of immunoreactive luteinizing hormone-releasing hormone (LHRH) from rat hypothalamus was examined using an in vitro superfusion system and sensitive assays for LHRH measurement. High extracellular (60 mM) K('+) ion repeatedly stimulated in vitro LHRH release, an effect attributed to nerve membrane depolarization triggering influx of Na('+) and Ca('++) ions and resulting in exocytosis of LHRH. K('+)-evoked LHRH release was strongly Ca('++)-dependent; however, Na('+) influx was not obligatory in depolarization-induced release. In support of calcium's role in LHRH release, agents permitting influx of Ca('++) (inophore A23187) or increased Ca('++) leakage from intracellular Ca('++) stores (2,4-dinitrophenol) stimulated LHRH release. K('+)-evoked release of LHRH is also thought to involve a microtubular transport mechanism, since colchicine (10('-6) or 10('-5) M) inhibited K('+)-stimulated LHRH output. However, 10('-4) M colchicine dramatically increased LHRH release as well as hypothalamic LHRH content by an unknown mechanism.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Physiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Hartter, Daryl Edward

• Biology, Animal Physiology

Identifier

(UMI)AAI8309952

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/71422