The Renin-Angiotensin System in The Freshwater Turtle Pseudemys Scripta

Initially, experiments were conducted to verify the existence of an endogenous renin-angiotensin system in this species. It was shown that Pseudemys scripta possess renal renin, renin substrate in the plasma, angiotensin converting enzyme activity, and vascular receptors specific for angiotensin. Kinetic analyses were performed on the turtle renal renin (kidney extract) plus turtle renin substrate (plasma) reaction, and the K(,m) for turtle renin determined. Comparing turtle angiotensin to mammalian {Asp('1), Ile('5), His('9)} angiotensin I on the basis of their biological, immunological, and chromatographic properties indicated that the primary structure of the turtle peptide is most likely different from that of mammalian angiotensin I. It was shown that a significant portion of turtle plasma renin may exist in an inactive form which can be made more active by in vitro acidification to pH 3.3 via dialysis.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Physiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Cipolle, Mark David

• Biology, Animal Physiology

Identifier

(UMI)AAI8309928

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/71421