Development of an Enzyme-Linked Immunosorbent Assay and Co-Agglutination Test for Diagnosis of Leptospirosis

An enzyme-linked immunosorbent assay (ELISA) for measuring leptospiral antibodies was developed. Desoxycholate extracted and type specific antigens of Leptospira interrogans serovars hardjo, szwajizak, pomona and grippotyphosa and sera from experimentally infected rabbits were used. No significant titer difference was observed when either horseradish peroxidase (HP) or alkaline phosphatase (AP) was used to label the anti immunoglobulin, although HP gave more sensitive reaction for early sera. When compared with the microscopic agglutination (MA) test, the ELISA with desoxycholate extracted antigens was more sensitive but showed more cross reactions. Type specific antigen of hardjo gave 80% sensitivity and 96.5% specificity in differentiating hardjo antibodies from that of other serovars in rabbits. The ELISA, therefore, should include the use of type specific antigens and HP-conjugated anti species immunoglobulin.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Veterinary Medical Science

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Budiharta, Setyawan

• Biology, Veterinary Science

Identifier

(UMI)AAI8409876

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/71310