Immunogenicity and Quantitation of Soluble Anaplasma Marginale Antigens From Cell Cultures and Blood

Short-term Anaplasma marginale cultures were initiated utilizing infected bovine blood from a splenectomized calf at various periods during acute parasitaemia employing Medium 199 supplemented with 10% adult bovine serum and 25 mM HEPES buffer. Better growth was observed in cultures initiated with lower percentage of parasitized erythrocytes (PPE) than those at higher PPE. Mineral supplementation (KCl and or MgSO(,4)) did not improve the ability of medium to support anaplasmal growth and appeared to be deleterious.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Veterinary Medical Science

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Moteane, Mohlalefi

• Health Sciences, Immunology

Identifier

(UMI)AAI8324610

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/71304