The Life Cycle and Pathogenicity of Isospora Suis in Gnotobiotic and Conventionalized Piglets

To study the life cycle and pathogenicity of I. suis infections, young (mostly 2-3 day old) germfree piglets were inoculated orally with 2.4 x 10('5) sporulated oocysts. The effects of a "normal" bacterial intestinal flora on the infection was determined by conventionalization of some germfree piglets with a pathogen-free bacterial preparation. Piglets were killed and necropsied at 3, 4, 5, 6, 8, 9, 10, 11, and 14 days post-inoculation (p.i.). The early stages of the life cycle were studied in four gnotobiotic piglets infected with dosages of up to 5 x 10('7) oocysts and sacrificed and 24 and 48 hours p.i. To study the possible presence of extra-intestinal stages, liver/spleen, and intestinal lymph node homogenates from two infected piglets, killed at 24 and 48 hours p.i., were injected intraperitoneally into two germfree piglets.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Veterinary Medical Science

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Harleman, Johannes Hendrikus

• Biology, Veterinary Science

Identifier

(UMI)AAI8302877

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/71298