Analysis of T-2 Toxin in Feed and Animal Specimens

A gas liquid chromatographic method was developed for the determination of T-2 toxin in feeds. The T-2 toxin was extracted from feeds with aqueous methanol. A zinc acetate and aluminum chloride solution was used to denature and precipitate proteinaceous compounds, plant pigments and other extraneous materials. The T-2 toxin was selectively partitioned into the benzene fraction, which was washed with 1% potassium hydroxide to remove acidic compounds. Other extraneous materials were removed by silica gel column chromatography using acetonitrile as an elutant. The acetonitrile was removed by evaporation and heptafluorobutyryl imidazole (HFBI) was added to form esters of the toxin. The esters were separated on a 2% OV-1, 6ft. glass column and quantitated with a Nickel-63 electron capture detector. The absolute recovery of the T-2 toxin spiked at concentrations between 0.5-7.0 ug/g was 100.62 + 10.24%. The proposed method could detect 0.1 ug/g in mixed feeds.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Veterinary Medical Science

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Ramaswamy, Venkatachalam

• Agriculture, Animal Pathology

Identifier

(UMI)AAI8209622

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/71292