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University of Illinois at Urbana-Champaign
Genetic and Biochemical Characterization of the Lipid-Protein Interactions of Pyruvate Oxidase
Abstract
dc:descriptionThe pyruvate oxidase structural gene (poxB) of Escherichia coli was cloned into derivatives of plasmid pBR322. The gene was first cloned into a cosmid vector by selection for the tetracycline resistance determinant of a closely linked Tn 10 insertion since no direct selection was available. Subsequent subcloning resulted in localization of the gene to a 3.2-kilobase-pair DNA segment, which was shown to cause the overproduction of oxidase activity (by six- to eightfold) and encode a protein having the size and antigenic determinants of pyruvate oxidase.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Microbiology
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2014
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Grabau, Charlotte Louise
Subjects
dc:subject × 1Identifiers
dc:identifier.*- Identifier
- (UMI)AAI8721643
- OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/71184