University of Illinois at Urbana-Champaign
The Structure of The Carbon Dioxide Reduction Factor, a Novel Carbon Carrier in Methanobacterium Thermoautotrophicum
Abstract
dc:descriptionCarbon dioxide reduction factor (CDR factor) was originally detected in a low-molecular-weight fraction of cell extract from Methanobacterium thermoautotrophicum that was required for methane production from CO(,2) by resolved cell extracts. The CDR factor of Romesser and Wolfe (Romesser, J. A., and Wolfe, R. S. (1982) Zbl. Bakt. Hyg., I. Abt. Orig. C 3:271-276) was separated into two components both of which were highly purified. One component was methanopterin (Keltjens, J. T., and Vogels, G. D. (1981) in Microbial Growth on C(,1) Compounds (Dalton, H., ed) pp. 152-158, Heydon and Son, Ltd., London), and for the other component the name CDR factor was retained. No known coenzymes tested substituted in the methane-producing assays for CDR factor and methanopterin, both of which were stable to boiling and exposure to air. The addition of CDR factor (0.8 (mu)g) and methanopterin (50 (mu)g) to the assay mixture increased by 12-fold the amount of methane formed from CO(,2).
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Microbiology
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2014
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Leigh, John Arthur
Subjects
dc:subject × 1Identifiers
dc:identifier.*- Identifier
- (UMI)AAI8409984