Characterization of Homogeneous and Heterogeneous Anti-Nucleic Acid Autoantibodies in Systemic Lupus Erythematosus

Three murine monoclonal anti-ssDNA autoantibodies were generated by fusion of spleen cells from autoimmune diseased NZB/NZW F1 hybrid mice with Sp 2/0-Ag 14 murine myeloma cell line. These monoclonal antibodies were purifed by affinity chromatography, and their immunochemical properties were studied using different methods. Specificity studies characterizing monoclonal anti-ssDNA autoantibodies revealed specificity for nucleotides. Clone E-4-1 showed preferential binding with mono, di, and polyguanosine. Clones E-4-4 and E-11-1 specifically bound mono, di, and polythymidine. Results of inhibition studies indicated the following order of reactivity for each monoclonal antibody: Polynucleotide > dinucleotide > mononucleotide. In a fluorescence immune assay, using aminonaphthyl derivatives of nucleotides, all three monoclonal antibodies exhibited low affinity for the homologous mononucleotides. The affinity of clone E-4-1 increased 10 folds when a dinucleotide derivative was used relative to a mononucleotide fluorescent probe.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Rezaei Poor Kardost, Robabeh

• Biology, Microbiology

Identifier

(UMI)AAI8203563

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/71148