Pathogenicity Genes of Pseudomonas Syringae Pv. Tabaci Br2

Pseudomonas syringae pv. tabaci BR2 produces tabtoxin and causes wildfire disease on tobacco and bean plants. Tn5 mutants of PTBR2.024 were generated by using pGS9::Tn5. The Tn5 mutants wre prototrophs, resistant to kanamycin, nalidixic acid, and streptomycin, but sensitive to chloramphenicol. Eight of 2,686 Tn5 mutants showed no symptoms and ten showed reduced symptoms when inoculated onto detached tobacco leaves of Nicotiana tabacum cv. Havana 38, but all showed typical wildfire symptoms on detached leaves of Phaseolus vulgaris cv. Top Crop. Two of the nonpathogenic mutants failed to produce tabtoxin. Southern hybridization analysis using ('32)P-labelled pGS9 indicated that Tn5 had inserted into different EcoRI and SalI restriction fragments in each of the eight nonpathogenic mutants. Hybridization to additional EcoRI and SalI bands in two mutants indicated a second insertion of IS50 or Tn5. The EcoRI fragment containing Tn5 from each of the eight nonpathogenic mutant genomic DNAs was cloned into vectors pTZ18R or pLAFR3.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Plant Pathology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Salch, Yangkyo Park

• Agriculture, Plant Pathology

Identifier

(UMI)AAI8711867

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/70609