Properties of Three Plasmids Isolated From Plant Pathogenic Pseudomonads

Indigenous plasmids isolated from Pseudomonas tabaci ATCC 11528 (pJP1), P. tabaci BR2 (pBPW1), and P. angulata 45 (pJP30) were labeled with Tn3 using RSF1010::Tn3 as a vector and the host strains were subsequently cured of their Tn3-containing plasmid. Plasmid-containing and respective cured strains produced nearly identical symptoms on host and nonhost plants when serial dilutions of bacteria were injected directly into leaf tissue. Also, cured and plasmid-containing isolates of P. tabaci 11528 and P. tabaci BR2 produced approximately the same amount of tabtoxin. These data indicate that pJP1, pBPW1, and pJP30 do not encode genes essential for pathogenicity and/or tabtoxin synthesis.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Plant Pathology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Obukowicz, Mark Gerard

• Agriculture, Plant Pathology

Identifier

(UMI)AAI8309993

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/70587