Sequence, Expression, and Mutagenesis of the Pseudomonas Putida Cytochrome P450(cam) Gene in Escherichia Coli

Cytochrome P450$\sb{\rm cam}$ catalyzes the stereospecific methylene hydroxylation of camphor to form 5-exo-hydroxycamphor and is encoded by the camC gene on the CAM plasmid of Pseudomonas putida, ATCC 17453. The cytochrome P450$\sb{\rm cam}$ structural gene has been cloned by mutant complementation in P. putida (Koga et al. 1985. Biochem. Biophys. Res. Commun. 130, 412-417). This investigation reports the complete nucleotide sequence of the camC gene along with 155 base pairs of 5$\sp\prime$ and approximately 790 base pairs of 3$\sp\prime$ flanking sequence. The gene encoding putidaredoxin reductase, camA, was located 22 nucleotides downstream from the camC gene. The camA gene initiated with a novel GUG codon, the first such initiator documented in Pseudomonas.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Unger, Benjamin Peter

Contributors dc:contributor

• Sligar, Stephen G.

• Biology, Molecular
• Chemistry, Biochemistry

Identifier

(UMI)AAI8823271

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/70571