Structure, Regulation and Comparative Biochemistry of the Pyrimidine Biosynthetic Gene Cluster in Bacillus Subtilis (Atcase)

A 10.5 kilobase PstI endonuclease fragment encoding the entire Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster was cloned in Escherichia coli. The cloned fragment also complemented E. coli pyrB, C, D, E, and F mutants. A restriction map of the cluster was generated, and many subclones were analyzed for their ability to complement E. coli pyr mutants. From these data a genetic map was generated; the gene order is pyrBCADFE. The B. subtilis pyrB gene was shown to be expressed in E. coli on the basis of immunochemical properties of the aspartate transcarbamylase (ATCase) activity in extracts.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Lerner, Claude George

• Chemistry, Biochemistry

Identifier

(UMI)AAI8711826

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/70561