Glycosyl Transferases in Chondroitin Sulfate Biosynthesis: Subcellular Location and Elongation Reaction

The subcellular location and polymerization reaction of the glucuronosyl transferase and the N-acetylgalactosaminyl transferase involved in the synthesis of chondroitin sulfate were examined in cultured embryonic chick tibial chondrocytes. The chondrocytes were subjected to subcellular fractionation by differential centrifugation and sucrose density gradient centrifugation. The glucuronosyl transferase, the N-acetylgalactosaminyl transferase, and also the sulfotransferases co-fractionated with the Golgi marker enzyme, galactosyl transferase. The specific activities and recoveries of the two glycosyl transferases in each fraction paralleled each other almost exactly.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Johnson, Jane Cutler

• Chemistry, Biochemistry

Identifier

(UMI)AAI8711815

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/70560