Phospholipid Transfer From Vesicles to High Density Lipoproteins, Catalyzed by Human Plasma Phospholipid Transfer Protein (Liposome)

Human plasma phospholipid transfer protein (PLTP) catalyzes the mass transfer of phosphatidylcholine (PC). Partial purification of PLTP yielded proteins with apparent M(,r) = 59,000 and 40,000 by SDS-PAGE. PLTP activity was measured by transfer of ('14)C L-(alpha)-dipalmitoyl PC from egg-PC vesicles to HDL. Activity was enhanced at low pH (4.5) and upon addition of (beta)-mercaptoethanol while Ca('+2) and Na('+) had no effect. E(,act) for facilitated PC transfer was 18.2 (+OR-) 2 kcal/mol.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Sweeny, Susan Anita

• Chemistry, Biochemistry

Identifier

(UMI)AAI8521890

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/70544