Chemotaxis Methyltransferase Ii From Bacillus Subtilis

Chemotaxis is the process by which motile cells sense and respond to their chemical environment. Following a period of time during which chemotactic bacteria respond to chemical stimulation, they revert to their pre-stimulus behavior, or adapt. Biochemically, adaptation is correlated with the methylation or demethylation of certain integral membrane proteins, the methyl-accepting chemotaxis proteins (MCPs). We have purified and characterized a chemotaxis methyltransferase (methyltransferase II) from Bacillus subtilis. By using a combination of DEAE Bio-Gel A chromatography, CM Bio-Gel A chromatography, ammonium sulfate fractionation, and S-adenosylhomocysteine affinity chromatography, a virtually homogeneous product was obtained.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Burgess-Cassler, Anthony

• Chemistry, Biochemistry

Identifier

(UMI)AAI8309917

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/70516