Design and Use of Fluorescent Assays to Study Plasminogen Activator and Other Serine Proteases Involved in Biological Control

In order to study the role of serine proteases in the wide variety of physiological functions which they serve, sensitive and quantitative assays need to be devised. Because the xanthene dyes rhodamine and fluorescein are among the most fluorescent compounds known and, as such, can be detected at very low concentrations, derivatives of these fluorescent dyes were designed as fluorogenic substrates for the amidase and esterase activity of serine proteases. As a consequence of their unique spectral and biochemical properties, these derivatives represent the most sensitive synthetic substrates for serine proteases yet described.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Leytus, Steven Phillip

• Chemistry, Biochemistry

Identifier

(UMI)AAI8218509

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/70507