Chemiluminescent Determinations of Clinical Analytes Using Microporous Membrane Flow Cells

The use of various chemiluminescence (CL) reactions as detection techniques for clinically important analytes has been explored in more depth over the last several years. This thesis project presented the use of microporous membrane flow cells for the chemiluminescent determinations of some of these species using flow injection analysis as an advantageous alternative to more classical methods of detection. The compounds studied were glucose, glactose, cholesterol (in both the free and esterified forms), creatinine, and creatine. Due to the lack of specificity of the CL system, an oxidase enzyme was used to convert the substrate (in the first three systems, the analyte of interest) into an equivalent amount of hydrogen peroxide, which in turn was quantitated by the luminol CL reaction. This oxidase enzyme was forced through a microporous membrane under pressure into an analyte stream that was flowing approximately three orders of magnitude faster. This approach had significant implications such as minimal enzyme consumption and negligible sample contamination by the enzyme, in addition to the fact that the enzymatic step occurred at its optimal pH simultaneously with the CL detection step at more basic pH.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Malavolti, Nathan Lee

• Chemistry, Analytical

Identifier

(UMI)AAI8623362

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/70331