The Role of Metal Ions and Metallo-Complexes in Luminol Chemiluminescence (Catalysis)

Luminol chemiluminescence (CL) has been employed for the sensitive detection of various analytes such as certain transition metal ions and hydrogen peroxide (H(,2)O(,2)). Metal ions and metallo-complexes significantly enhance the light output of the luminol CL reaction (these species are generally referred to as catalysts in the literature). Unfortunately, the mechanism responsible for this enhancement is unknown. This research project studied the metallo-catalyzed liminol CL reaction to improve the understanding of this reaction. Photon emission, extent of reaction, fluorescence efficiency and other related measurements were obtained for the luminol reaction as a function of catalyst identity and concentration. The metallo-catalysts studied were hemin, hemoglobin, horseradish peroxidase (HRP), ferricyanide, Co(II), Cu(II), Cr(III), Fe(II), Fe(III), Ni(II) and Mn(II). In contrast to most reports in the literature, these measurements allowed the direct determination of quantum yield of CL ((PHI)(,CL)) values.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Nekimken, Howard Lewis

• Chemistry, Analytical

Identifier

(UMI)AAI8610965

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/70319