Part One: Investigation of the Immunogenicity of a Spin Label Probe. Part Two: An Electron Paramagnetic Resonance Study of Dinitrophenyl-Binding Myelomas and Hybridomas Using Spin Labelled Probes

In Part One, the spin label 2,2,5,5-tetramethylpyrrilidin-3-carboxyl-1-oxyl (tmcpo) was investigated for its immunogenicity at attempting covalent attachment of the probe to a carrier molecule. Detectable immune responses to these immunogens in New Zealand adult rabbits could not be observed with either capillary precipitin tests, immunoelectrophoresis, or epr studies. Fluorescamine was used to quantitate the substitution of the spin probe on BSA, and results indicated the spin probe was leaching from the carrier. Epr studies confirmed the leaching phenomenum. Formation of an ammonium carboxylate ion pair was proposed rather than the anticipated amide bond.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Oester, Dean Alan

• Chemistry, Biochemistry

Identifier

(UMI)AAI8422788

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/70263