Classical and Modern Genetic Manipulations for Improving Butanol Production by Clostridium Acetobutylicum in Extruded Corn Broth (Tolerance, Transformation, Dnase)

By employing serial enrichment, a derivative of Clostridium acetobutylicum ATCC 824 was obtained which grew at concentrations of butanol that prevented growth of the wild-type strain. The SA-1 mutant produced consistently higher concentrations of butanol (from 5 to 14%) and lower concentrations of acetone (12.5 to 40%) than the wild-type strain in 4-20% extruded corn broth (ECB). SA-1 demonstrated higher conversion efficiency of extruded corn to butanol than the wild type strain at every concentration of ECB tested. Characterization of the wild-type and SA-1 strain in 6% ECB demonstrated the superiority of the latter in terms of growth rate, time of onset of butanol production, carbohydrate utilization, pH resistance, and final butanol concentration in the fermentation broth.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Food Science

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Lin, Yun-Long

• Biology, Microbiology

Identifier

(UMI)AAI8422118

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/70075