Methods to Induce and Synchronize Ovulation and to Enhance Corpus Luteum Function and Fertility in Beef Females (Postpartum Cows, Heifers)

A competitive inhibition double antibody enzyme immunoassay (EIA) for determining plasma levels of progesterone in beef females was developed using a horseradish peroxidase labelled progesterone conjugate and polysterene tubes. The sensitivity of the assay was 5 pg and 84% of the progesterone spiked to samples was recovered, which was similar to recovery of tritiated progesterone spiked samples (83%). The first antibody (anti 11-alpha-hydroxy progesterone hemisuccinate : bovine serum albumin) had a high specificity for progesterone and the intraassay and interassay coefficients of variation of pooled samples were 7.9% and 13.8%, respectively. Samples assayed by both RIA and EIA were in excellent agreement (r = 0.96).

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Animal Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Do Valle, Ezequiel R.

• Biology, Animal Physiology
• Agriculture, Animal Culture and Nutrition

Identifier

(UMI)AAI8610918

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/70034