The Use of Delayed Implantation Model in The Study of Hormonal Requirement for Implantation in The Rat

A delayed implantation rat model has been developed. Method of ovariectomy was described. Ovariectomy could be performed up to the morning of day 4, beyond that ovariectomy was not effective. Injection of Tamoxifen (10 or 50 (mu)g/200 g BW) in the afternoon of either day 4 or day 5 inhibited implantation. Subcutaneous injection of 1.0 microgram of estrone induced implantation in 100% of the delayed implantation rats used, while lower (0.1 (mu)g/200 g BW) and higher doses (5.0 (mu)g/200 g BW) resulted in fewer implantation. Similarly, diethylstilbestrol induced implantation when given at doses of 0.5 and 1.0 (mu)g/200 g BW. The more effective dose, 0.5 (mu)g/200 g BW resulted in only 50% of rats implanted.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Animal Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Nasution, Syahrun Hamdani

• Biology, Animal Physiology

Identifier

(UMI)AAI8324611

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/70008