Gene Amplification for the Improved Secretion of Foreign Proteins in the Yeast Saccharomyces Cerevisiae

Gene amplification plays a central role in maximizing yield for protein production. Generally, more copies of a gene generally lead to an increase in protein production. The 2μ plasmid of Saccharomyces cerevisiae has been used extensively as a vector for foreign protein expression because of its natural amplification to 60-100 copies per cell. Integration of genes into the chromosomes provides the advantage of increasing stability over 2μ based vectors. Integration vectors allow integration of multiple copies of a gene leading to improved protein production.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemical Engineering

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Shaw, Mark Robert

Contributors dc:contributor

• Wittrup, K. Dane

• Biology, Molecular
• Biology, Genetics
• Engineering, Chemical

Identifier

(UMI)AAI9624492

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/69800