Human Neuroblastoma, Strain Sk-N-Sh, as a Model System of Nerve Cell Differentiation: Neuronal Development, Plasminogen Activator Release, and Unscheduled Deoxyribonucleic Acid Synthesis

This study characterized morphological and biochemical properties of the cultured human neuroblastoma, strain SK-N-SH, maintained in vitro. In complete growth medium supplemented with 15% fetal bovine serum, SK-N-SH cells exhibited a large nucleus, with several distinct nucleoli, and neurite- and dendrite-like processes with branched end terminals. Within a few hours after subcultivation, the morphological complexity of the cells increased as evidenced by a greater number of cells with neurite processes longer than 50 (mu)m, and an increased elongation of the processes already present. Two to six percent of the cells had at least one process greater than 50 (mu)m in length on day 1 after plating, and this increased to fifteen to thirty-five percent on days 3 or 4. The mean total summed process length per cell increased (TURN)4.7-fold between days 1 and 2.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Physiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Blakely, William Francis

• Biology, Animal Physiology

Language dc:language

eng

Identifier

(UMI)AAI8119633

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/68312