Mechanisms of Immunosuppression in Murine Malaria

An extract was prepared from Plasmodium berghei, NK65C strain, by disrupting P. berghei infected erythrocytes with a Parr bomb disruption cell. This extract was capable of reducing the anti-DNP and SRBC plaque forming cell response of mice intraperitoneally injected with the extract one day prior to immunization. The suppressive substance of this crude extract was precipitated with 50% saturated ammonium sulfate (SAS) but not with 33% SAS. Further purification of the suppressor factor was possible by means of gel filtration chromatography on Sephadex G-150 using sodium borate buffer containing 6M guanidine hydrochloride. The purified soluble suppressive factor was tentatively characterized as a small glycoprotein (M.W. 27,000 daltons), possessing isoelectric point of 7.00. The suppressor activity could be abrogated by proteolytic enzyme digestion but was not abolished by periodate oxidation. The purified suppressor substance did not reduce the PFC response to DNP-Ficoll, a T-independent antigen, but it was capable of reducing the T-dependent anti-DNP and anti-SRBC PFC responses in high dilution. The data obtained from these experiments suggested that B cells are not the target of suppression.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Veterinary Medical Science

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Khansari, Nematollah

• Biology, Veterinary Science

Language dc:language

eng

Identifier

(UMI)AAI8114442

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/68236