Effect of Lipid Modification on Transformation - Associated Properties and Viral Membrane Assembly

We have developed the methodology to systematically alter the phospholipid and fatty acid composition of the cellular membranes in chicken embryo fibroblasts. We have used this technique to study the effect of lipid modification on various parameters associated with transformation in tissue culture. Lipid supplementation had a variety of effects on cellular morphology, adhesion, cell surface proteins, and cell growth. These studies led to the observation that the standard lipid composition of the plasma membrane between normal and transformed cells was different. It was found that the transformed cells had a more rigid plasma membrane, as measured by DPH fluorescence polarization. The increased polarization was correlated with a higher phosphatidylethanolamine/phosphatidylcholine ratio in the transformed cell plasma membrane, as well as differences in the fatty acid composition.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Pessin, Jeffrey Eugene

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(UMI)AAI8114459

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/67409