Purification and Characterization of Simian Virus 40 Large T Antigen

Simian Virus 40 is a small DNA virus which can replicate normally following productive infection of permissive monkey cells, transform various cell lines of different species in tissure culture, and induce tumor formation following injection into newborn hamsters. Large T antigen is one of two viral-coded non-structural proteins, and is produced early in productive infection and continuously in transformed cells. Several lines of evidence indicate that large T antigen of SV40 is involved in the initiation of viral DNA replication during productive infection, the stimulation of host cell DNA synthesis following SV40 infection, the regulation of viral transcription, and the initiation and maintenance of transformation. In order to better understand its role in the above processes, large T antigen was purified from a line of SV40 transformed human fibroblasts (SV80 cells) and its biochemical activities studied.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Giacherio, Donald Allen

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(UMI)AAI8108516

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/67403