Application of Microporous Membranes to Chemiluminescence Analysis

A novel approach to reagent introduction into flowing streams is accomplished using a microporous membrane to separate a reagent reservoir from an analyte stream. Evidence is presented to support the contention that upon pressurization of the reagent reservoir a locally high concentration of reagents is established near the surface of the membrane in the analyte stream. Using reagent flow rates of only a few (mu)L/min, reagent introduction using microporous membranes is shown to provide high sensitivity and good reproducibility when tested using the luminol-cobalt (II)-hydrogen peroxide chemiluminescence system. The limit of detection for cobalt(II) is about 10('-10) M (10 parts per trillion); typical precision is (+OR-) 3%. The advantages of using microporous membranes for chemiluminescence analysis are discussed and possible areas of future application are described.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Nau, Vance James, Jr.

• Chemistry, Analytical

Language dc:language

eng

Identifier

(UMI)AAI8114451

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/67263